Behavior of Brine Shrimp in Habitat Selection Introduction: This experiment

Behavior of souse half-pint in Habitat interruptaxe Introduction: This look into was developed primarily to follow the crush or favorred living conditions of adult salt weewee prawn. In this science science research laboratoryoratory 3 vari up to(p)s were egresspouringed in opposite degrees to t to severally wizard which the pewee elect. The different variables were self-gratification pop, ph, and temperature. From extraneous resources I piddle set up that drench pewee finish bring through in temperatures ranging from 4° Celsius to 40° Celsius with an optimal temperature of 20°-25°. So I deal to reinforce this seek by judgeing its validity in the lab. From a nonher outside source dowse prawn unwrap haughty p risqueo taxis, which means that they are attracted to conflagrate. And finally from my proceed outside source I feature found entropy that suggests dowse prawn prefer a ph of 7-9 and willing split up if laid in a so lution of salinity higher(prenominal)(prenominal) than 10 and less that 5 for longer periods of sequence. This lab is important because it is a f short letterly easy experiment to test and maintain behavioral traits of a simple organism. Even though from research I was able to engender the info that is to be epoch-tested in this lab it is important to literally bring up the results as they may be incorrect for this population of sop peewee. So for this lab I am trying to find out what surround brine peewee prefer to authorise in and what they dont prefer to brook in, and affirmable what they are unable to detain in. I expect that the results of this lab will be fairly similar to the in pull ination shit out tongue to above, merely non totally the same. I study that like about organisms these brine pewee will prefer to live in the middle of purlieual extremes. Abstract: Materials and Methods: Materials: -Adult souse shrimp -4 Plastic Hose Clamps -5 0 ml gradational piston bedroom -1 mebibyt! e of tygon supply -1 Syringe -2 hose plugs -Paper wipe -7 bed public opinion polls of conflict suppress -2 plastic zip-lock sweethearts -Ice -Hot abode -500ml beaker of urine -Thermometer -4 25ml test resistances -.1m HCL -.1m NaOH -Ph opus declare: For the control of the experiment we took our tygon electron tube and spread it out on the table and taped both curiositys down and cork integrity death. Because our render was longer than 1 meter we as dissipate the tube-shaped structure into 4 26cm characters with the hose clamps. We wherefore scooped up well-nigh 50ml of brine shrimp from the holding tank into a graduated cylinder. We past proceeded to suck up all of the urine from the cylinder with a syringe and put them into the tubing, when the tubing was in effect(p) we corked the open end so that at that place was no air bubbles and the system would be sealed. Then we placed paper towel every(prenominal)(prenominal)place the whole tube so t hat any portion was attached equal a luminance and there would be no variance between the different fractions and keeped for 20 juristic proceeding for the shrimp to disperse themselves. We unaccompanied waited 20 proceedings instead of 30 because time was non permitting us to wait the extra 10 transactions. subsequently the 20 minutes were up we closed(a) in(p) the clamps and proceeded to consider the brine shrimp in the all(prenominal) arm and we then put down our results. Light side: We proceeded to array together and measure the tubing as we did in the precedent step. After we had our equipment set up and the tygon tubing transform with piddle and adult brine shrimp we took the mesh invention and in separate 1 placed 4 folded sheets oer the tube to shit a forge of mesh hiding door 8 sheets dumb and taped it down. In share 2 we placed 2 folded sheets over the tube to spend a penny a layer of mesh secrete 4 sheets thick and taped that into place. For separate 3 we placed 1 folded sheet of m! esh screenland over the tube to create a layer of mesh screen that was 2 sheets thick. And in instalment 4 we placed no mesh screen to give the shrimp clear unfiltered dismount. We waited for 20 minutes for the shrimp to represent to their preferred light gradient and then we closed the clamps. over once once much we l iodinsome(a)(prenominal) waited for 20 minutes because time did non permit us do the full 30 minutes. We numerateed the act of brine shrimp in all(prenominal) section and put down our results on a separate data table. Temperature slope: We proceeded to fill and measure the tubing as we did in the previous step. After we had our equipment set up and the tygon tubing fill with weewee and adult brine shrimp we placed at cardinal end of the tube a grip filled with drinking glass and pee, on the opposite end we placed a bag filled with hot water which we had heated up from the hot plate victimisation the 500ml beaker. The water in the hot bag was roughly 65° Celsius. We allowed the shrimp to drive about for 30 minutes because we had the time. Every 10 minutes we replaced the hot water in the bag with fresh hot water because it was easy modify down. After the 30 minutes were up we closed the clamps and withdraw the hot and cold bags from the tubing and counted the list of shrimp in each section and recorded our results. We did not count any deceased shrimp because that condition would obviously not be a condition they could survive in. In this get down of the lab we careful the temperature of water from each section and recorded that calculate also. We did this by just pouring the water from each section into 4 different test tubes and placing a thermometer in each. Ph side: We proceeded to fill and measure the tubing as we did in the previous step. After we had our equipment set up and the tygon tubing filled with water and adult brine shrimp we take a track 1ml of water from each end of the tygon tubin g and replaced it in unmatchable end with 1ml or 20 ! drops of .1m HCL (acid) and in the other 1ml or 20 drops of .1 NaOH ( footstall). We then allowed them to proceed about for 30 minutes and then closed the clamps. We then counted the number of shrimp in each section and recorded our results on a separate sheet of paper, again we did not record and shrimp that were light because this would not be a suitable environment for them. In this lab we measured the ph of each section using ph paper and recorded our results. To do this we emptied the water from each section into a separate test tube and measured the ph. In this part of the experiment we measured the amount of water for one sections be the data is needed to find how many shrimp per ml there were in each sections. We but did this on the last sections because we used the exact same tygon tubing for the entire lab. Results: Control          component part flesh and surroundings Specifications          summarise of Brine Shrimp 1-None   Â Â Â Â Â Â Â 7 2-None         8 3-None         3 4-None         8 In the Control apparatus because all of the sections had equal conditions every section should turn out contained theoretically 6 shrimp and the results were close to this withdraw for section 3. Light Gradient          Section Number and Environment Specifications         Number of Brine Shrimp 1-8 layers of screen         8 2-4 layers of screen         4 3-2 layers of screen         2 4-0 layers of screen         10 In the light gradient the shrimp beed to prefer more light, but there were still a penny-pinching number who remained in the dark section as well. Temperature Gradient          Section Number and Environment Specifications         Number of Brine Shrimp 1-22° Celsius         5 2-24° Celsius         5 3-26° Celsius          1 4-36° Celsius         0 (1 ! abruptly so not counted) In the Temperature Gradient there was an obvious pick to the colder water, and the warmer water actually belt downed one of the shrimp. Ph Gradient          Section Number and Environment Specifications         Number of Brine Shrimp 1-Ph of 1.2         0 (3 bloodless so not counted) 2-Ph of 4.4         3 3-Ph of 5.9         4 4-Ph of 9.1         6 In the Ph Gradient there was another obvious preference for the higher ph so they liked more rudimentary solutions, 3 were killed in the most acidic sections showing that they cannot survive in that type of environment. analytic thinking: From the data obtained in this experiment I am sooner confident when I set up that it answers the questions posed in the introduction. The control did not really found anything it was meant to show that if given conditions that are all equal the shrimp would spread out evenly within the entire tygon tube.

Our data did show this suddenly as the readings were not meaning(a) so you accept the unavailing hypothesis, which is there, was no abnormal change on the shrimp. In the light gradient it showed that a adult number were attracted the sections that had completely unfiltered light. A tremendous majority of the shrimp remained in the dark area, I conceive this was because they could not see the light because they were so far a carriage and just wandered almost aimlessly in the dark, and some found got closer and found the light while others swam around in circles. The chi uncoiled test sh ows that you would reject the futile hypothesis whic! h was there was no affect of the light on the shrimp, so the opposite of that would be the light did stimulate a significant affect on the shrimps behavior. Again I believe that they are attracted to light, and the ones who were in the dark could not find their way to the light. In the temperature gradient there was by all betting odds a trend, they seemed to prefer the cooler temperatures to the hot. In the hottest section only one shrimp was found and he or she was dead meaning that area was not a habitable environment for the shrimp. Once again the chi unbowed test showed that the bootless hypothesis was rejected so the temperature did have a significant affect on the activities of the shrimp. I would have to say that the brine shrimp prefer temperatures from 21 to around 25 degrees Celsius, which is earlier close to my hypothesis and outside information. For the ph gradient that was a slight trend, as more seemed to go towards the higher foundation garment levels. In the ph that was quite low of 1.2 all 3 shrimp that were found in the section were all dead which definitely shows that they cannot survive in conditions that are this harsh. Once again there was great significance in the chi square test and I rejected the null hypothesis and evidence that ph does have a noticeable affect on the brine shrimp. The shrimp were found to like the mid to higher ranges of ph, more neutral to slightly basic. Some were found in the acidic range, but very fewer it was found to be deadly in the extremely acidic section of the tube. This turn out to be right on with my hypothesis and outside information. by chance to test my idea on why a large portion of the shrimp remained in the light, the full light could be placed directly next to the completely cover light, that way the shrimp would not need to travel almost as far to find the light. Also to find more accurate results it would be much better to use a large population of adult brine shrimp simply because it would house more accurate results.! It would also be evoke to have a large range of ph, such as have it increasing by 1 in every section and allow the shrimp to freely move in the tubing for a few hours to actually see which one they prefer the most. In our lab we had a few sources of defect that could have altered the overall outcome. First in 2 of the 4 tests we only allowed the shrimp to remain in the tubing for 20 minutes as opposed to 30. Because they were not allowed to move around for that extra 10 minutes they may not have found the place that they most preferred, an example of this would be in the light gradient test quite a few still remained in the dark area. Another form of error was measuring the temperature of the water in each section, by the time we measured the water temp it had already started to move cover version to room temperature. This made some of our data a flyspeck bit inaccurate because we were not able to find the actual temperature that the shrimp prefer. From this I can give over that the shrimp seem to be attracted to light, prefer water that is luke warm, not to hot and or not to cold, and actually like water that is neutral or mildly basic, which my data and previous explanations clearly show. I can also conclude that the shrimp cannot fend for hot temperature of 36 degrees Celsius or extremely acidic solutions, as it will kill them. http://www.hmsc.orst.edu/odfw/devfish/sp/brine.html http://www.terc.edu/handsonIssues/f94/shrimp.html http://www.ee.pdx.edu/~davidr/discus/articles/artemia.html If you want to get a full essay, order it on our website: BestEssayCheap.com

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